Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of xylose and glucose into ethanol and other products

被引:51
作者
Liu, Siqing [1 ]
Skinner-Nemec, Kelly A. [1 ]
Leathers, Timothy D. [1 ]
机构
[1] USDA, Agr Res Serv, Natl Ctr Agr Utilizat Res, Bioprod & Biocatalysis Res Unit, Peoria, IL 61604 USA
关键词
ethanol tolerance; mixed sugar fermentation; xylose; Phenotype Microarray; Lactobacillus buchneri; lactate;
D O I
10.1007/s10295-007-0267-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lactobacillus buchneri strain NRRL B-30929 was isolated from a fuel ethanol production facility. This heterofermentative, facultative anaerobe can utilize xylose as a sole carbon source and tolerates up to 12% ethanol. Carbohydrate utilization (API, Biomerieux) and Phenotype Microarrays (TM) (PM, Biolog) analyses indicated that the strain is able to metabolize a broad spectrum of carbon sources including various monosaccharides (C5 and C6), disaccharides and oligosaccharides, with better rates under anaerobic conditions. In pH-controlled bioreactors, the bacterium consumed xylose and glucose simultaneously at high concentrations (125 g L-1, pH 6.0). The major fermentation products were lactate (52 g L-1), acetate (26 g L-1) and ethanol (12 g L-1). The strain ferments glucose alone (pH 4.0) into lactate and ethanol with a molar ratio of 1.03:1. This strain will be further explored via genetic engineering for potential applications in biomass conversion.
引用
收藏
页码:CP4 / 81
页数:7
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