Research of qualitative ELISA and GC-MS for Atrazine in soil

被引：2

作者：

Hu Xiaohang ^{[1
]}

Wang Xiwei ^{[1
]}

Zhou Qin ^{[1
]}

Wu Yumei ^{[1
]}

Xu Qingxuan ^{[1
]}

机构：

[1] Heilongjiang Univ, Crop Acad, Harbin, Peoples R China

来源：

ENVIRONMENTAL BIOTECHNOLOGY AND MATERIALS ENGINEERING, PTS 1-3 | 2011年 / 183-185卷

关键词：

soil; atrazine; ELISA; GC-MS;

D O I：

10.4028/www.scientific.net/AMR.183-185.990

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The qualitative ELISA was used for screening out atrazine in soil and positive results were proved with GC-MS. The recoveries,Precision and the detection limits were estimated in this paper. The average recoveries of ELISA were 96.64%similar to 98.06%,and coefficient of variations were 1.69%similar to 3.36%,the detection limit was 0.04 mu g/kg, while The average recoveries of GC-MS were 85.78%similar to 87.46%,and coefficient of variations were 4.3%similar to 6.56%,the detection limit was 0.03 mu g/kg, when the concentrations of 0.5,1.0,5.0 mu g/kg atrazine standard were add to soil samples. Then three positive samples that filtered by ELISA were proved with GC-MS, and the results were coherent between two methods. The assay found ELISA is a rapid and exact method with good reproducibility for atrazine determination in soil

引用

页码：990 / 993

页数：4

共 7 条

[1]

ELISA and HPLC methods for atrazine and simazine determination in trophic chains samples [J].