Exosomes Transfer Among Different Species Cells and Mediating miRNAs Delivery

被引:61
作者
Zhou, Yueyuan [1 ]
Tian, Tian [2 ]
Zhu, Yanliang [1 ]
Ali, Doulathunnisa Jaffar [1 ]
Hu, Feihu [1 ]
Qi, Yuhua [3 ]
Sun, Bo [1 ]
Xiao, Zhongdang [1 ]
机构
[1] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Dept Neurobiol, 101 Longmian Avenu, Nanjing 211166, Jiangsu, Peoples R China
[3] Jiangsu Ctr Dis Prevent & Control CDC, Microbiol Lab, Minist Hlth, Key Labs Enter Pathogen Microbiol, 172 Jiangsu Rd, Nanjing 210009, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
EXOSOMES; MIR-21; PTEN; ACROSS-SPECIES; INTESTINAL EPITHELIAL-CELLS; PTEN; CANCER; MICRORNAS; MICROVESICLES; PROLIFERATION; ACCUMULATION; SUPPRESSION; PROTEINS; VESICLES;
D O I
10.1002/jcb.26077
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Exosomes, the natural vehicles of intercellular communication, transfer proteins, mRNAs, and microRNAs (miRNAs) and mediate many physiological and pathological processes. It is not clear that whether exosomal miRNAs could regulate gene expression across species, though some studies suggest interactions of exosomal miRNAs between cells. In this report, we have isolated exosomes from rat PC12 cells and assessed their internalization by human cancer Hela cells. The internalized exosomes were located in Hela lysosomes. Human PTEN expression was significantly deregulated due to miR-21 delivered by rat cell exosomes. Our results prove that exosomes could incorporate between cells of different species and could regulate the protein expressions in the recipient cells by delivering the enclosed miRNAs. Thus our study foreshadows a futuristic treatment approach of utilizing miRNA enclosed exosome vehicles sans species concerns in combating various diseases/ regulating abnormal proteins. J. Cell. Biochem. 118: 4267-4274, 2017. (c) 2017 Wiley Periodicals, Inc.
引用
收藏
页码:4267 / 4274
页数:8
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