New approach to the synthesis of oligodeoxyribonucleotides modified with phosphorothioates of predetermined sense of P-chirality

被引：13

作者：

Nawrot, B ^{[1
]}

Rebowska, B ^{[1
]}

Cieslinska, K ^{[1
]}

Stec, WJ ^{[1
]}

机构：

[1] Polish Acad Sci, Ctr Mol & Macromol Studies, Dept Bioorgan Chem, PL-90363 Lodz, Poland

来源：

TETRAHEDRON LETTERS | 2005年 / 46卷 / 39期

关键词：

P-chiral dinucleoside phosphorothioate; oligonucleotide phosphorothioate; PS-DNA; S-protection; oligonucleotide synthesis;

D O I：

10.1016/j.tetlet.2005.07.158

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Appropriately protected, diastereomerically pure dinucleoside phosphorothioates, obtained by the stereocontrolled oxathiaphospholane method, were S-alkylated with 2-nitrobenzyl bromide and then converted into their 3 '-0-phosphoramidites. The corresponding S-protected dinucleotide building blocks were successfully used for the synthesis of oligonucleotides containing P-stereodefined phosphorothioate bonds at preselected positions. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：6641 / 6644

页数：4

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