Virus inactivation of blood products by phenothiazine dyes and light

被引：68

作者：

Mohr, H ^{[1
]}

Bachmann, B ^{[1
]}

KleinStruckmeier, A ^{[1
]}

Lambrecht, B ^{[1
]}

机构：

[1] GERMAN RED CROSS,TRANSFUS SERV,INST SPRINGE,SPRINGE,GERMANY

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1997年 / 65卷 / 03期

关键词：

D O I：

10.1111/j.1751-1097.1997.tb08586.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methylene blue (MB) and its derivatives azure A, B, C and thionine are photoactive and, in principle, are suitable for photodynamic virus inactivation of blood and blood products, such as therapeutic plasma. Methylene blue was selected for plasma decontamination because it is being clinically used and because of its known toxicological and other properties, The standard procedure for photodynamic treatment of single units of fresh plasma involves illumination with visible light at an MB concentration of 1 mu M. Polymerase chain reaction analysis revealed that, in addition to model viruses, the bloodborne viruses hepatitis B virus, hepatitis C virus, human immune deficiency virus-1 and probably also the nonenveloped parvovirus B19 are sensitive to MB/light treatment, The procedure is further improved when the fluorescent tubes routinely used for illumination are replaced by more intense light sources, e.g. light-emitting diodes or low-pressure sodium lamps, Surprisingly, the improved virus kill is accompanied by reduced damage to plasma proteins.

引用

页码：441 / 445

页数：5

共 18 条

[1]

[Anonymous], 1965, PHOTOCHEM PHOTOBIOL, DOI DOI 10.1111/J.1751-1097.1965.TB05733.X

[2] TARGET STRUCTURES FOR HIV-1 INACTIVATION BY METHYLENE-BLUE AND LIGHT [J].

BACHMANN, B ;

KNUVERHOPF, J ;

LAMBRECHT, B ;

MOHR, H .

JOURNAL OF MEDICAL VIROLOGY, 1995, 47 (02) :172-178

[3]

BENHUR E, 1995, PHOTOCHEM PHOTOBIOL, V62, P353

[4] SEQUENCE-ANALYSIS OF THE 5' NONCODING REGION OF HEPATITIS-C VIRUS [J].

BUKH, J ;

PURCELL, RH ;

MILLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4942-4946

[5]

CHANG TW, 1975, P SOC EXP BIOL MED, V148, P291

[6]

HEINMETS F, 1955, AIR5355 WR W REED AR

[7] PHOTOINACTIVATION OF VIRUSES IN HUMAN FRESH PLASMA BY PHENOTHIAZINE DYES IN COMBINATION WITH VISIBLE-LIGHT [J].

LAMBRECHT, B ;

MOHR, H ;

KNUVERHOPF, J ;

SCHMITT, H .

VOX SANGUINIS, 1991, 60 (04) :207-213

[8]

LILLIE RD, 1990, H J CONS BIOL STAINS, P415

[9] AGAR SUSPENSION CULTURE FOR SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS [J].

MACPHERSON, I ;

MONTAGNIER, L .

VIROLOGY, 1964, 23 (02) :291-&

[10] Hepatitis agent cloned and designated hepatitis G virus [J].

McCarthy, M .

LANCET, 1996, 347 (8997) :313-313

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