Neutron reflectometry of membrane protein assemblies at the solid/liquid interface

被引：9

作者：

Holt, SA ^{[1
]}

Lakey, JH

Daud, SM

Keegan, N

机构：

[1] Rutherford Appleton Lab, ISIS, CCLRC, Chilton OX11 0QX, England

[2] Newcastle Univ, Sch Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

来源：

AUSTRALIAN JOURNAL OF CHEMISTRY | 2005年 / 58卷 / 09期

关键词：

D O I：

10.1071/CH05112

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Neutron reflectometry has been used to study the self-assembly of a membrane protein, OmpF, onto a gold-coated silicon substrate from solution. OmpF associates into trimers and has been modified so that each trimer binds to the gold substrate through cysteine residues. Quantitative analysis of the data revealed that 12% of the surface was covered by the oriented protein with 27600 water molecules surrounding each trimer.

引用

页码：674 / 677

页数：4

共 13 条

[1] FORMATION OF MONOLAYER FILMS BY THE SPONTANEOUS ASSEMBLY OF ORGANIC THIOLS FROM SOLUTION ONTO GOLD [J].

BAIN, CD ;

TROUGHTON, EB ;

TAO, YT ;

EVALL, J ;

WHITESIDES, GM ;

NUZZO, RG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (01) :321-335

[2] Growth of highly ordered thin silicate films at the air-water interface [J].

Brown, AS ;

Holt, SA ;

Reynolds, PA ;

Penfold, J ;

White, JW .

LANGMUIR, 1998, 14 (19) :5532-5538

[3] Neutron and X-ray reflectometry: Solid multilayers and crumpling films [J].

Brown, AS ;

Holt, SA ;

Saville, PM ;

White, JW .

AUSTRALIAN JOURNAL OF PHYSICS, 1997, 50 (02) :391-405

[4] THE STRUCTURE OF OMPF PORIN IN A TETRAGONAL CRYSTAL FORM [J].

COWAN, SW ;

GARAVITO, RM ;

JANSONIUS, JN ;

JENKINS, JA ;

KARLSSON, R ;

KONIG, N ;

PAI, EF ;

PAUPTIT, RA ;

RIZKALLAH, PJ ;

ROSENBUSCH, JP ;

RUMMEL, G ;

SCHIRMER, T .

STRUCTURE, 1995, 3 (10) :1041-1050

[5] CRYSTAL-STRUCTURES EXPLAIN FUNCTIONAL-PROPERTIES OF 2 ESCHERICHIA-COLI PORINS [J].

COWAN, SW ;

SCHIRMER, T ;

RUMMEL, G ;

STEIERT, M ;

GHOSH, R ;

PAUPTIT, RA ;

JANSONIUS, JN ;

ROSENBUSCH, JP .

NATURE, 1992, 358 (6389) :727-733

[6] DETERGENT STRUCTURE IN TETRAGONAL CRYSTALS OF OMPF PORIN [J].

PEBAYPEYROULA, E ;

GARAVITO, RM ;

ROSENBUSCH, JP ;

ZULAUF, M ;

TIMMINS, PA .

STRUCTURE, 1995, 3 (10) :1051-1059

[7]

PENFOLD J, 1991, N-HOLLAND D, P223

[8] Neutron reflectivity and soft condensed matter [J].

Penfold, J .

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2002, 7 (1-2) :139-147

[9] Recent advances in the study of chemical surfaces and interfaces by specular neutron reflection [J].

Penfold, J ;

Richardson, RM ;

Zarbakhsh, A ;

Webster, JRP ;

Bucknall, DG ;

Rennie, AR ;

Jones, RAL ;

Cosgrove, T ;

Thomas, RK ;

Higgins, JS ;

Fletcher, PDI ;

Dickinson, E ;

Roser, SJ ;

McLure, IA ;

Hillman, AR ;

Richards, RW ;

Staples, EJ ;

Burgess, AN ;

Simister, EA ;

White, JW .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1997, 93 (22) :3899-3917

[10]

ROSENBUSCH JP, 1974, J BIOL CHEM, V249, P8019

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