Chromosome instability is a predominant trait of fibroblasts from Li-Fraumeni families

被引:41
作者
Boyle, JM
Mitchell, ELD
Greaves, MJ
Roberts, SA
Tricker, K
Burt, E
Varley, JM
Birch, JM
Scott, D
机构
[1] Christie CRC Res Ctr, CRC Dept Canc Genet, Paterson Inst Canc Res, Manchester M20 9BX, Lancs, England
[2] Christie CRC Res Ctr, CRC Dept Biomath, Manchester M20 9BX, Lancs, England
[3] Christie CRC Res Ctr, Paediat & Familial Canc Res Grp, Manchester M20 9BX, Lancs, England
关键词
Li-Fraumeni; p53; senescence; radiation sensitivity; chromosome aberrations;
D O I
10.1038/bjc.1998.364
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Previous work has indicated a role for p53 in cell cycle control, genomic stability and cellular responses to DNA-damaging agents. However, few data are available for human fibroblasts heterozygous for defined germline mutations in TP53. We report studies on 25 strains derived from 12 families with Li-Fraumeni syndrome (LFS) and 18 strains from normal volunteers. The families include th ree that are classical LFS families, but in whom no TP53 mutation has been found. In the families with mutations, increased longevity and resistance to low-dose-rate ionizing radiation showed a statistically significant association with the presence of TP53 mutations. However, not all heterozygotes had increased longevity or were radioresistant, and fibroblasts from cancer-affected members of LFS families without TP53 mutations showed no significant increase in either of these end points. In contrast, all mutation-carrying strains showed evidence of genomic instability expressed as aneuploidy, and accumulated structural chromosome aberrations in up to 100% of cells, usually accompanied by loss of the wild-type TP53 allele, immediately before senescence. Levels of aneuploidy higher than in normal cells were also observed in fibroblasts from families without TP53 mutations, suggesting that chromosome instability is a major factor in determining the cancer proneness of these families.
引用
收藏
页码:2181 / 2192
页数:12
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