Neutrophil activation and chemotaxis after in vitro treatment with perfluorocarbon

被引：51

作者：

Rossman, JE ^{[1
]}

Caty, MG ^{[1
]}

Rich, GA ^{[1
]}

Karamanoukian, HL ^{[1
]}

Azizkhan, RG ^{[1
]}

机构：

[1] SUNY BUFFALO, CHILDRENS HOSP, SCH MED & BIOMED SCI, DEPT PEDIAT SURG, BUFFALO, NY USA

来源：

JOURNAL OF PEDIATRIC SURGERY | 1996年 / 31卷 / 08期

关键词：

perfluorocarbon; neutrophils; chemotaxis; activation;

D O I：

10.1016/S0022-3468(96)90105-0

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

The in vitro effects of perfluorocarbon (PFC) on human neutrophil activation and chemotaxis were examined. Neutrophils were incubated, with and without PFC, and were analyzed for chemotaxis through 5-mu m and 8-mu m pore filters. Neutrophil activation was quantitated by flow cytometry. Activation studies showed that PFC-treated neutrophil samples (n = 6) produced only 39.83% +/- 25.9% (mean +/- SD) of matched control (n = 6) fluorescence. Chemotaxis studies showed that PFC-treated neutrophil samples (n = 8) had migration of only 18.63% +/- 6.5% (of control values) of neutrophils to the outer boundary of the 5-mu m filters in = 8 controls). The 8-mu m pore filter migration (n = 8) was similarly low, with a mean outer boundary migration count of only 26% +/- 19.8% of the control (n = 8) value. Thus, neutrophils exposed to perfluorocarbon produce significantly less detectable H2O2 (P < .001) and have a significantly lower chemotactic response (P < .001). Copyright (C) 1996 by W.B. Saunders Company

引用

页码：1147 / 1150

页数：4

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