Rapid determination of chloramphenicol residues in aquaculture tissues by immunochromatographic assay

被引：27

作者：

Li, Ke ^{[1
]}

Liu, Liqiang

Xu, ChuanLai

Chu, XiaoGang

机构：

[1] WuXi kangBaiEr Biotechnol Corp, Wuxi 214011, JiangSu, Peoples R China

[2] So Yangtze Univ, Sch Food Sci & Technol, WuXi 214036, JiangSu, Peoples R China

[3] Chinese Acad Inspect & Quarantine, Beijing 100082, Peoples R China

来源：

ANALYTICAL SCIENCES | 2007年 / 23卷 / 11期

关键词：

D O I：

10.2116/analsci.23.1281

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An immunochromatographic assay was developed to detect chloramphenicol (CAP) residues in aquaculture tissues. The limit of detection (LOD) was 10 ng g(-1) for detecting CAP spiked in the aquaculture tissues. The results were confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS) and indicated that there was a good agreement between the two methods. The linear regression equation was y = 1.19x + 0.539 with R-2 = 0.978. The assay time for test was less than 5 min and the method is suitable for rapid testing on-site.

引用

页码：1281 / 1284

页数：4

共 20 条

[1] DOT-ELISA FOR THE RAPID DETECTION OF GENTAMICIN IN MILK [J].

ARA, J ;

GANS, Z ;

SWEENEY, R ;

WOLF, B .

JOURNAL OF CLINICAL LABORATORY ANALYSIS, 1995, 9 (05) :320-324

[2] Chemiluminescence enzyme immunoassay (CLEIA) for the determination of chloramphenicol residues in aquatic tissues [J].

Chuanlai, X ;

Peng, CF ;

Hao, K ;

Jin, ZY ;

Wang, WK .

LUMINESCENCE, 2006, 21 (02) :126-128

[3] HYBRIDOMA CELL-LINE PRODUCTION OF A SPECIFIC MONOCLONAL-ANTIBODY TO THE MYCOTOXINS ZEARALENONE AND ALPHA-ZEARALENOL [J].

DIXON, DE ;

WARNER, RL ;

RAM, BP ;

HART, LP ;

PESTKA, JJ .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1987, 35 (01) :122-126

[4]

HARLOW ED, 1988, COLD SPRING HARBOR L, P196

[5]

HEITZMAN RJ, 1994, VET DRUG RESIDUES, P127

[6]

HUANG SL, 1991, ACTA AGR NUCL SINICA, V12, P291

[7] ENZYME IMMUNOASSAYS OF KANAMYCIN GROUP ANTIBIOTICS WITH HIGH SENSITIVITIES USING ANTI-KANAMYCIN AS A COMMON ANTI-SERUM - REASONING AND SELECTION OF A HETEROLOGOUS ENZYME LABEL [J].

KITAGAWA, T ;

FUJIWARA, K ;

TOMONOH, S ;

TAKAHASHI, K ;

KOIDA, M .

JOURNAL OF BIOCHEMISTRY, 1983, 94 (04) :1165-1172

[8] Competitive ELISA of chloramphenicol: Influence of immunoreagent structure and application of the method for the inspection of food of animal origin [J].

Kolosova, AY ;

Samsonova, JV ;

Egorov, AM .

FOOD AND AGRICULTURAL IMMUNOLOGY, 2000, 12 (02) :115-125

[9] Enzyme immunoassay using peroxidase as a label and a dip-strip test for monitoring residual bacitracin in chicken plasma [J].

Matsumoto, M ;

Tsunematsu, K ;

Tsuji, A ;

Kido, Y .

ANALYTICA CHIMICA ACTA, 1997, 346 (02) :207-213

[10] SIMULTANEOUS DETERMINATION OF THIAMPHENICOL, FLORFENICOL, AND CHLORAMPHENICOL RESIDUES IN MUSCLES OF ANIMALS AND CULTURED FISH BY LIQUID-CHROMATOGRAPHY [J].

NAGATA, T ;

SAEKI, M .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1992, 15 (12) :2045-2056

← 1 2 →