A putative integrase gene defines the distal end of a large cluster of ToxR-regulated colonization genes in Vibrio cholerae

被引:124
作者
Kovach, ME [1 ]
Shaffer, MD [1 ]
Peterson, KM [1 ]
机构
[1] LOUISIANA STATE UNIV, MED CTR, DEPT MICROBIOL & IMMUNOL, SHREVEPORT, LA 71130 USA
来源
MICROBIOLOGY-SGM | 1996年 / 142卷
关键词
Vibrio cholerae; colonization; integrase; ToxR regulon; mobile genetic element;
D O I
10.1099/13500872-142-8-2165
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A large cluster of virulence genes encoding proteins involved in Vibrio cholerae accessory colonization factor (ACF) expression and toxin-coregulated pilus (TCP) biogenesis is flanked by sequences that resemble bacteriophage attachment (att) half-sites. Adjacent to the attL-like site is a gene (int) that encodes a protein related to the integrase family of site-specific recombinases. The putative vibrio integrase appears to be most closely related to the Escherichia coli cryptic prophage (CP4-57) integrase protein (52% identity, 73% similarity). Genomic analysis of numerous V. cholerae strains (01, non-01 and 0139) revealed that only vibrios capable of causing epidemic Asiatic cholera possess the TCP-ACF colonization gene cluster in association with the integrase. The fact that the integrase gene is absent in avirulent strains suggests that epidemic strains of V. cholerae obtained the TCP-ACF colonization gene cluster via horizontal transfer.
引用
收藏
页码:2165 / 2174
页数:10
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