Ethanol production and fermentation characteristics of recombinant Saccharomyces cerevisiae strains grown on starch

被引:53
作者
Birol, G
Önsan, ZI
Kirdar, B
Oliver, SG
机构
[1] Bogazici Univ, Dept Chem Engn, TR-80815 Bebek, Istanbul, Turkey
[2] UMIST, Dept Biochem & Appl Mol Biol, Manchester M60 1QD, Lancs, England
关键词
recombinant yeast; starch; fermentation; alpha-amylase; glucoamylase;
D O I
10.1016/S0141-0229(97)00244-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The production of ethanol from starch has been investigated in three genetically modified Saccharomyces cerevisiae strains (YPG/AB, YPG/MM, and YPB-G). Two of the three strains produce the Aspergillus awamori glucoamylase together with either the Bacillus subtilis (YPG/AB) or the mouse (YPG/MM) alpha-amylase as separately secreted polypeptides. YPB-G, on the other hand, secretes a bifunctional fusion, protein that contains both the B. subtilis alpha-amylase and the A. awamori glucoamylase activities. Substrate utilization, biomass growth, and ethanol production were all studied in both starch- and glucose-containing media. Much higher growth rates were found when any of the three strains were grown on glucose. YPG/AB showed the most efficient utilization of starch for ethanol production with the lowest levels of reducing sugars accumulating in the medium. The superior performance of YPG/AB as compared to YPB-G was found to correlate with its higher level of alpha-amylase activity. The ethanol production levels of YPG/AB in starch- and glucose-containing media were found to be comparable. YPB-G, which secretes the bifunctional fusion protein, could produce ethanol in media with starch concentrations above 100 g l(-1) while YPG/M(M) over dot did not produce ethanol from starch because of its negligible secretion of glucoamylase. (C) 1998 Elsevier Science Inc.
引用
收藏
页码:672 / 677
页数:6
相关论文
共 13 条
[1]  
ASTOLFI S, 1986, BIO-TECHNOL, V4, P311
[2]   AMYLASES, ALPHA AND BETA [J].
BERNFELD, P .
METHODS IN ENZYMOLOGY, 1955, 1 :149-158
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   DEVELOPMENT OF YEAST STRAINS FOR THE EFFICIENT UTILIZATION OF STARCH - EVALUATION OF CONSTRUCTS THAT EXPRESS ALPHA-AMYLASE AND GLUCOAMYLASE SEPARATELY OR AS BIFUNCTIONAL FUSION PROTEINS [J].
DEMORAES, LMP ;
ASTOLFI, S ;
OLIVER, SG .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1995, 43 (06) :1067-1076
[5]   QUANTITATIVE CHARACTERIZATION OF PLASMID INSTABILITY IN SACCHAROMYCES-CEREVISIAE USING FLOW CYTOMETRY-CELL SORTING [J].
HJORTSO, MA ;
DENNIS, KE ;
BAILEY, JE .
BIOTECHNOLOGY LETTERS, 1985, 7 (01) :21-24
[6]   THE STABILITY OF THE YEAST PLASMID PJDB248 DEPENDS ON GROWTH-RATE OF THE CULTURE [J].
KLEINMAN, MJ ;
GINGOLD, EB ;
STANBURY, PF .
BIOTECHNOLOGY LETTERS, 1986, 8 (04) :225-230
[7]   EFFECT OF AMINO-ACIDS ON GROWTH AND PHOSPHATE-METABOLISM IN A PROTOTROPHIC YEAST-STRAIN [J].
LUDWIG, JR ;
OLIVER, SG ;
MCLAUGHLIN, CS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 79 (01) :16-23
[8]   EFFECT OF GROWTH-RATE AND EXPRESSION LEVEL ON PLASMID STABILITY IN SACCHAROMYCES-CEREVISIAE [J].
PARKER, C ;
DIBIASIO, D .
BIOTECHNOLOGY AND BIOENGINEERING, 1987, 29 (02) :215-221
[9]   CONSTRUCTION OF AN ALPHA-AMYLASE GLUCOAMYLASE FUSION GENE AND ITS EXPRESSION IN SACCHAROMYCES-CEREVISIAE [J].
SHIBUYA, I ;
TAMURA, G ;
SHIMA, H ;
ISHIKAWA, T ;
HARA, S .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (06) :884-889
[10]   CLONING OF THE ALPHA-AMYLASE CDNA OF ASPERGILLUS-SHIROUSAMII AND ITS EXPRESSION IN SACCHAROMYCES-CEREVISIAE [J].
SHIBUYA, I ;
TAMURA, G ;
ISHIKAWA, T ;
HARA, S .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (02) :174-179