Benzo(a)pyrene induces interleukin (IL)-6 production and reduces lipid synthesis in human SZ95 sebocytes via the aryl hydrocarbon receptor signaling pathway

被引：31

作者：

Hu, Tingting ^{[1
]}

Pan, Zhanyan ^{[2
]}

Yu, Qian ^{[1
]}

Mo, Xiaohui ^{[1
]}

Song, Ningjing ^{[1
]}

Yan, Min ^{[2
]}

Zouboulis, Christos C. ^{[3
,4
,5
]}

Xia, Longqing ^{[2
]}

Ju, Qiang ^{[2
]}

机构：

[1] Anhui Med Univ, Clin Acad, Shanghai Dermatol Hosp, Dept Dermatol, Shanghai 200443, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Dept Dermatol, Shanghai 200127, Peoples R China

[3] Dessau Med Ctr, Dept Dermatol, D-14195 Dessau, Germany

[4] Dessau Med Ctr, Dept Venereol, D-14195 Dessau, Germany

[5] Dessau Med Ctr, Dept Allergol & Immunol, D-14195 Dessau, Germany

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2016年 / 43卷

基金：

中国国家自然科学基金;

关键词：

SZ95; sebocytes; Benzo(a)pyrene; AhR; IL-6; Acne; Acne inversa; ENVIRONMENTAL CONTAMINANT; EXPRESSION; AHR; PATHOGENESIS; ACTIVATION; ACNE;

D O I：

10.1016/j.etap.2016.02.011

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

In this study, we determined the effects of benzo(a)pyrene (BaP) on the expression of aryl hydrocarbon receptor (AhR) and cytochrome P450 1A1 (CYP1A1), and assessed the action of BaP on inflammatory cytokine expression and lipid synthesis in SZ95 sebocytes in vitro. BaP (10(-8), 10(-7), 10(-6) and 10(-5) M) was not cytotoxic for SZ95 sebocytes after 24 h exposure. Expression of AhR was promoted in mRNA lever, while was inhibited in protein lever after BaP (10(-5) M) exposure. CYP1A1 expression was up-regulated in both mRNA and protein levels. BaP (10(-5) M) exerted a stimulatory action on interleukin (IL)-6 secretion, while a dose-dependently inhibitory effect on lipid synthesis from 10(-8) M to 10(-5) M in SZ95 sebocytes. Both actions were partly antagonized in AhR-knockdowned SZ95 sebocytes. This study demonstrates that BaP can activate AhR signaling pathway, and exhibits pro-inflammatory effects and inhibitory effects on sebum production in human sebocytes. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：54 / 60

页数：7

共 28 条

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