Provitamin A Accumulation in Cassava (Manihot esculenta) Roots Driven by a Single Nucleotide Polymorphism in a Phytoene Synthase Gene

被引:203
作者
Welsch, Ralf [1 ]
Arango, Jacobo [1 ]
Baer, Cornelia [1 ]
Salazar, Bertha [1 ]
Al-Babili, Salim [1 ]
Beltran, Jesus [2 ]
Chavarriaga, Paul [2 ]
Ceballos, Hernan [2 ]
Tohme, Joe [2 ]
Beyer, Peter [1 ]
机构
[1] Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany
[2] Ctr Int Agr Trop, Cali, Colombia
基金
比尔及梅琳达.盖茨基金会;
关键词
SEED-SPECIFIC OVEREXPRESSION; BETA-CAROTENE; ABSCISIC-ACID; PLANTS; BIOSYNTHESIS; YELLOW; MAIZE; RICE; INHIBITION; EXPRESSION;
D O I
10.1105/tpc.110.077560
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount of provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accumulation to fight vitamin A deficiency disorders. We show here that an allelic polymorphism in one of the two expressed phytoene synthase (PSY) genes is capable of enhancing the flux of carbon through carotenogenesis, thus leading to the accumulation of colored provitamin A carotenoids in storage roots. A single nucleotide polymorphism present only in yellow-rooted cultivars cosegregates with colored roots in a breeding pedigree. The resulting amino acid exchange in a highly conserved region of PSY provides increased catalytic activity in vitro and is able to increase carotenoid production in recombinant yeast and Escherichia coli cells. Consequently, cassava plants overexpressing a PSY transgene produce yellow-fleshed, high-carotenoid roots. This newly characterized PSY allele provides means to improve cassava provitamin A content in cassava roots through both breeding and genetic modification.
引用
收藏
页码:3348 / 3356
页数:9
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