Homologue structure of the SLAC1 anion channel for closing stomata in leaves

被引:111
作者
Chen, Yu-hang [1 ,2 ]
Hu, Lei [3 ]
Punta, Marco [2 ,4 ,5 ]
Bruni, Renato [2 ]
Hillerich, Brandan [2 ]
Kloss, Brian [2 ]
Rost, Burkhard [1 ,2 ,4 ,5 ]
Love, James [2 ]
Siegelbaum, Steven A. [3 ,6 ,7 ]
Hendrickson, Wayne A. [1 ,2 ,7 ,8 ]
机构
[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA
[2] New York Struct Biol Ctr, NYCOMPS, New York, NY 10027 USA
[3] Columbia Univ, Dept Neurosci, New York, NY 10032 USA
[4] Tech Univ Munich, Dept Comp Sci, D-85748 Munich, Germany
[5] Tech Univ Munich, Inst Adv Study, D-85748 Munich, Germany
[6] Columbia Univ, Dept Pharmacol, New York, NY 10032 USA
[7] Columbia Univ, Howard Hughes Med Inst, New York, NY 10032 USA
[8] Columbia Univ, Dept Physiol & Cellular Biophys, New York, NY 10032 USA
关键词
ABSCISIC-ACID; CHLORIDE CHANNEL; GUARD-CELL; PLASMA-MEMBRANE; SULFITE EFFLUX; PROTEIN; SELECTIVITY; MODEL; GENE; CONSERVATION;
D O I
10.1038/nature09487
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The plant SLAC1 anion channel controls turgor pressure in the aperture-defining guard cells of plant stomata, thereby regulating the exchange of water vapour and photosynthetic gases in response to environmental signals such as drought or high levels of carbon dioxide. Here we determine the crystal structure of a bacterial homologue (Haemophilus influenzae) of SLAC1 at 1.20 angstrom resolution, and use structure-inspired mutagenesis to analyse the conductance properties of SLAC1 channels. SLAC1 is a symmetrical trimer composed from quasi-symmetrical subunits, each having ten transmembrane helices arranged from helical hairpin pairs to form a central five-helix transmembrane pore that is gated by an extremely conserved phenylalanine residue. Conformational features indicate a mechanism for control of gating by kinase activation, and electrostatic features of the pore coupled with electrophysiological characteristics indicate that selectivity among different anions is largely a function of the energetic cost of ion dehydration.
引用
收藏
页码:1074 / U157
页数:9
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