Selection for transgene homozygosity in embryonic stem cells results in extensive loss of heterozygosity

被引：35

作者：

Lefebvre, L ^{[1
]}

Dionne, N

Karaskova, J

Squire, JA

Nagy, A

机构：

[1] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[2] Univ Toronto, Dept Med Genet, Toronto, ON M5G 1X5, Canada

来源：

NATURE GENETICS | 2001年 / 27卷 / 03期

基金：

加拿大健康研究院;

关键词：

D O I：

10.1038/85808

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Embryonic stem cells offer unprecedented opportunities for random or targeted genome alterations in the mouse. We present here an efficient strategy to create chromosome-specific loss of heterozygosity in embryonic stem cells. The combination of this method with genome-wide mutagenesis in ES cells (using chemical mutagens or gene-trap vectors) opens up the possibility for in vitro or in vivo functional screening of recessive mutations in the mouse.

引用

页码：257 / 258

页数：2

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