Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes

被引:112
作者
Jaruga, E
Salvioli, S
Dobrucki, J
Chrul, S
Bandorowicz-Pikula, J
Sikora, E
Franceschi, C
Cossarizza, A
Bartosz, G
机构
[1] Univ Lodz, Dept Mol Biophys, PL-90237 Lodz, Poland
[2] Univ Modena, Sch Med, Dept Biomed Sci, Modena, Italy
[3] Jagiellonian Univ, Dept Biophys, Lab Confocal Microscopy & Image Anal, Krakow, Poland
[4] Med Univ Lodz, Lab Flow Cytometry, Lodz, Poland
[5] M Nencki Inst Expt Biol, PL-02093 Warsaw, Poland
[6] INRCA Ancona, Dept Gerontol Sci, Ancona, Italy
来源
FEBS LETTERS | 1998年 / 433卷 / 03期
关键词
apoptosis; curcumin; membrane asymmetry; permeability; mitochondrial membrane potential;
D O I
10.1016/S0014-5793(98)00919-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Curcumin (diferuoylmethane) is a natural compound with anticarcinogenic activities which is able to exert either proapoptotic or antiapoptotic effects in different cell types, This paper focuses on the sequence and extent of primary events induced by curcumin, in comparison with those occurring during dexamethasone-induced apoptosis in rat thymocytes, It also presents annexin VI-FITC as a new probe for studying membrane asymmetry. Curcumin readily penetrates into the cytoplasm, and is able to accumulate in membranous structures such as plasma membrane, endoplasmic reticulum and nuclear envelope. Curcumin-treated cells exhibit typical features of apoptotic cell death, including shrinkage, transient phosphatidylserine exposure, increased membrane permeability and decrease in mitochondrial membrane potential. However, nuclei morphology, DNA fragmentation, the extent and time-course of membrane changes are different from those observed during dexamethasone-induced apoptosis, suggesting that, despite many similarities, the mode of action and the events triggered by curcumin are different from those occurring during typical apoptosis, (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:287 / 293
页数:7
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