Microcystin-leucine arginine (MC-LR) induced inflammatory response in bovine sertoli cell via TLR4/NF-κB signaling pathway

被引：33

作者：

Adegoke, E. O. ^{[1
]}

Wang Chen ^{[1
]}

Machebe, N. S. ^{[2
]}

Wang Xue ^{[1
]}

Wang Hao ^{[1
]}

Adeniran, S. O. ^{[1
]}

Zhang Han ^{[1
]}

Zheng Peng ^{[1
]}

Zhang Guixue ^{[1
]}

机构：

[1] Northeast Agr Univ, Coll Anim Sci & Technol, Dept Anim Genet Breeding & Reprod, Harbin, Heilongjiang, Peoples R China

[2] Univ Nigeria, Dept Anim Sci, Nsukka, Nigeria

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2018年 / 63卷

关键词：

Bovine; Sertoli cells; Apoptosis; Inflammation; Toxicity; EPITHELIAL BARRIER FUNCTION; TUMOR-NECROSIS-FACTOR; TOLL-LIKE RECEPTORS; NF-KAPPA-B; TIGHT JUNCTION; INTERFERON-GAMMA; IN-VITRO; IMMUNE-RESPONSES; INNATE IMMUNITY; RAT TESTIS;

D O I：

10.1016/j.etap.2018.09.001

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

Sertoli cells were treated with 0, 20, 40, 60 and 80 mu g/L of MC-LR to investigate its toxic effects, mechanism of action and immune response of the cells. Our results revealed that treatment containing 20 mu g/L of MC-LR was non-toxic to the cells. Treatments containing 40, 60 and 80 mu g/L of MC-LR reduced the cell viability, induced nuclear morphological changes and downregulated the blood-testis barrier constituent proteins within 48 h after treatment. The toll-like receptor 4 (TLR4) and nuclear factor-kappaB (NF-kappa B) were activated and significantly (P < 0.05) upregulated in cells treated with 40, 60 and 80 mu g/L of MC-LR compared to the control. The pro inflammatory cytokines were upregulated within 48 h after treatment. However commencing from 72 h, upregulation of anti-inflammatory cytokines and expression of blood-testis barrier constituent proteins was observed. This study indicates that MC-LR induced inflammatory response in bovine Sertoli cell via activation of TLR4/NF-kappa B signaling pathway.

引用

页码：115 / 126

页数：12

共 74 条

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Selenium (Na2SeO3) Upregulates Expression of Immune Genes and Blood-Testis Barrier Constituent Proteins of Bovine Sertoli Cell In Vitro [J].