Detection of virus-like particles in liver biopsies from HCV-infected patients

被引：19

作者：

Bosman, C

Valli, MB

Bertolini, L

Serafino, A

Boldrini, R

Marcellini, M

Carloni, G

机构：

[1] CNR, IMS, Area Ric Tor Vergata, Electron Microscopy Lab, I-00133 Rome, Italy

[2] Univ Rome La Sapienza, Sch Med, Dept Expt Med & Pathol, Rome, Italy

[3] IRCCS, Casa Sollievo Sofferenza, Foggia, Italy

[4] CNR, Inst Expt Med, Rome, Italy

[5] CNR, Inst Cell Biol, Rome, Italy

[6] IRCCS, Paediat Hosp Bambino Gesu, Rome, Italy

来源：

RESEARCH IN VIROLOGY | 1998年 / 149卷 / 05期

关键词：

HCV; virus-like particles; infected livers;

D O I：

10.1016/S0923-2516(99)89011-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In order to directly ascertain the presence of HCV virus infection in livers of patients with HCV chronic hepatitis, we investigated, by transmission electron microscopy (TEM), liver biopsies from 2 adults and 4 children for the presence of virus-like particles (VLPs). The plasmas of these HCV-positive patients were HCV-RNA-positive, with high ALT values. In liver tissue samples examined, we were able to detect plus and minus strands of HCV RNA by strand-specific RT-PCR. Aggregates or single VLPs of about 45 nm in diameter were detectable in variable amounts in endoplasmic cisternae and in hepatocyte cytoplasms of infected patients. These results emphasize the relevance of performing TEM assays to confirm the diagnosis of HCV infection.

引用

页码：311 / 314

页数：4

共 15 条

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