Modulation by topiramate of AMPA and kainate mediated calcium influx in cultured cerebral cortical, hippocampal and cerebellar neurons

被引：85

作者：

Poulsen, CF

Simeone, TA

Maar, TE

Smith-Swintosky, V

White, HS

Schousboe, A

机构：

[1] Danish Univ Pharmaceut Sci, Dept Pharmacol, DK-2100 Copenhagen, Denmark

[2] Univ Utah, Dept Pharmacol & Toxicol, Program Neurosci, Salt Lake City, UT 84112 USA

[3] Johnson & Johnson Pharmaceut Res & Dev LLC, CNS Res, Drug Discovery, Spring House, PA USA

来源：

NEUROCHEMICAL RESEARCH | 2004年 / 29卷 / 01期

关键词：

desensitization; receptors; glutamate; N-methyl-D-aspartate; NMDA;

D O I：

10.1023/B:NERE.0000010456.92887.3b

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The effect of the antiepileptic drug topiramate on Ca2+ uptake through (RS)-2-amino-3-(3-hydroxy-5-methylisoxazol-4-yl)propionate (AMPA) and kainate (KA) receptors was investigated in different cell culture systems consisting of neurons from the cerebral cortex, hippocampus, and cerebellum. Ca2+ influx was assayed using a fluorescent Ca2+ chelator to monitor changes in the intracellular Ca2+ concentration or cobalt staining to assess the effect of topiramate on Ca2+-permeable AMPA/KA receptors. In all types of neuronal cultures studied, AMPA and KA were found to elicit an influx of Ca2+ in a subset of the neuronal population. Topiramate, at concentrations of 30 and 100 muM, inhibited Ca2+ influx by up to 60%. Modulation of AMPA and KA-evoked Ca2+ influx may contribute to both the antiepileptic and neuroprotective properties of topiramate.

引用

页码：275 / 282

页数：8

共 46 条

[1]

DREJER J, 1987, J NEUROSCI, V7, P2910

[2]

SELECTION OF A PURE CEREBELLAR GRANULE CELL-CULTURE BY KAINATE TREATMENT [J].