Hydrolytic DNA cleavage promoted by a dinuclear iron(III) complex

被引：82

作者：

Neves, A ^{[1
]}

Terenzi, H

Horner, R

Horn, A

Szpoganicz, B

Sugai, J

机构：

[1] Univ Fed Santa Catarina, Dept Quim, Lab Bioinorgan & Cristalog, BR-88040900 Florianopolis, SC, Brazil

[2] Univ Fed Santa Catarina, Dept Bioquim, Lab Expessao Genica, BR-88040900 Florianopolis, SC, Brazil

[3] Univ Fed Santa Catarina, Dept Quim, Lab Equilibrio Quim, BR-88040900 Florianopolis, SC, Brazil

[4] Univ Fed Santa Catarina, Dept Bioquim, Lab Mutagenicidade, BR-88040900 Florianopolis, SC, Brazil

来源：

INORGANIC CHEMISTRY COMMUNICATIONS | 2001年 / 4卷 / 08期

关键词：

DNA cleavage; dinuclear iron(III) complex; artificial nuclease; drug-DNA interaction;

D O I：

10.1016/S1387-7003(01)00233-7

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The dinuclear iron complex [Fe-2(III)(BPClNOL)(2)(H2O)(2)](ClO4)(2) (1) [N-(2-hydroxybenzyl)-N-(pyridylmethyl)[(3-chloro)(2-hydroxy)] propylamine] was investigated focusing on its hydrolytic activity towards phosphate esters and DNA. The activity was evaluated within the pH range 6.1-8.0 when the hydroxo molecules are coordinated to the Fe-III centers of(1). The complex did not show hydrolytic activity on the 4-nitrophenyl phosphate and bis(2,4-dinitrophenyl)phosphate. However, the complex successfully promoted the hydrolytic cleavage of plasmid DNA, producing single and double DNA strand breaks. (C) 2001 Elsevier Science B.V, All rights reserved.

引用

页码：388 / 391

页数：4

共 13 条

[1] DESIGN OF A DOUBLE-STRANDED DNA CLEAVING AGENT WITH 2 POLYAMINE METAL-BINDING ARMS - RU(DIP)2MACRON+ [J].