8Br-cGMP mediates relaxation of tracheal smooth muscle through PKA

被引：20

作者：

Algara-Suárez, P ^{[1
]}

Espinosa-Tanguma, R ^{[1
]}

机构：

[1] Univ Autonoma San Luis Potosi, Fac Med, Dept Fisiol & Farmacol, San Luis Potosi 78210, Mexico

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 314卷 / 02期

关键词：

PKA; PKG; cGMP; BKCa; channels;

D O I：

10.1016/j.bbrc.2003.12.136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, guinea pig tracheal smooth muscle pre-contracted with histamine was relaxed by the addition of 100 muM 8Br-cGMP, a non-hydrolyzable and cell-permeable analog for cGMP. This effect was not sensitive to cGMP-dependent protein kinase (PKG) inhibitors, whereas it was partially blocked by cAMP-dependent protein kinase (PKA) inhibitors. The relaxation observed was also reverted up to 50 +/- 8.5% by iberiotoxin, a selective inhibitor of large conductance, calcium-activated potassium channels (BKCa). Our results indicate that there exists a crosstalk mechanism between cAMP and cGMP signaling pathways which lead to relaxation of guinea pig tracheal smooth muscle and also that BKCa channels are involved to a certain extent in this phenomenon. (C) 2003 Elsevier Inc. All rights reserved.

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页码：597 / 601

页数：5

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