Structure of synaptophysin: A hexameric MARVEL-domain channel protein

被引：60

作者：

Arthur, Christopher P. ^{[1
]}

Stowell, Michael H. B. ^{[1
]}

机构：

[1] Univ Colorado, MCD Biol, Boulder, CO 80309 USA

来源：

STRUCTURE | 2007年 / 15卷 / 06期

关键词：

D O I：

10.1016/j.str.2007.04.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synaptophysin I (SypI) is an archetypal member of the MARVEL-domain family of integral membrane proteins and one of the first synaptic vesicle proteins to be identified and cloned. Most all MARVEL-domain proteins are involved in membrane apposition and vesicle-trafficking events, but their precise role in these processes is unclear. We have purified mammalian SypI and determined its three-dimensional (3D) structure by using electron microscopy and single-particle 3D reconstruction. The hexameric structure resembles an open basket with a large pore and tenuous interactions within the cytosolic domain. The structure suggests a model for Synaptophysin's role in fusion and recycling that is regulated by known interactions with the SNARE machinery. This 3D structure of a MARVEL-domain protein provides a structural foundation for understanding the role of these important proteins in a variety of biological processes.

引用

页码：707 / 714

页数：8

共 34 条

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