Combined enzymatic synthesis of nucleotide (deoxy) sugars from sucrose and nucleoside monophosphates

被引：33

作者：

Zervosen, A ^{[1
]}

Stein, A ^{[1
]}

Adrian, H ^{[1
]}

Elling, L ^{[1
]}

机构：

[1] UNIV DUSSELDORF,INST ENZYME TECHNOL,RES CTR JULICH,D-52404 JULICH,GERMANY

来源：

TETRAHEDRON | 1996年 / 52卷 / 07期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0040-4020(95)01081-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The synthesis of NDP-glucose 3a-d (N = A, C, U, dU) with sucrose synthase B was combined with the enzymatic synthesis of nucleoside diphosphates 2a-d from their corresponding nucleoside monophosphates 1a-d by different kinases A. Further combination with recombinant dTDP-glucose 4,6-dehydratase D enabled us to synthesize dUDP-6-deoxy-alpha-D-xylo-4-hexulose 5 from 1d on a preparative scale. By using the repetitive batch technique the enzymatic syntheses of nucleotide (deoxy) sugars 3a-d, 5 could be realized on a 0.1 - 0.5 g scale.

引用

页码：2395 / 2404

页数：10

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