Xyn10A, a Thermostable Endoxylanase from Acidothermus cellulolyticus 11B

被引：28

作者：

Barabote, Ravi D. ^{[2
]}

Parales, Juanito V. ^{[1
]}

Guo, Ying-Yi ^{[2
]}

Labavitch, John M. ^{[2
]}

Parales, Rebecca E. ^{[1
]}

Berry, Alison M. ^{[2
]}

机构：

[1] Univ Calif Davis, Dept Microbiol, Davis, CA 95616 USA

[2] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 21期

关键词：

BACTERIUM THERMOTOGA-MARITIMA; INDUSTRIAL APPLICATIONS; MICROBIAL XYLANASES; BINDING DOMAIN; PURIFICATION; ENZYMES; CELLULOSE; FUNGI; XYNA;

D O I：

10.1128/AEM.01326-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We cloned and purified the major family 10 xylanase (Xyn10A) from Acidothermus cellulolyticus 11B. Xyn10A was active on oat spelt and birchwood xylans between 60 degrees C and 100 degrees C and between pH 4 and pH 8. The optimal activity was at 90 degrees C and pH 6; specific activity and K-m for oat spelt xylan were 350 mu mol xylose produced min(-1) mg of protein(-1) and 0.53 mg ml(-1), respectively. Based on xylan cleavage patterns, Xyn10A is an endoxylanase, and its half-life at 90 degrees C was approximately 1.5 h in the presence of xylan.

引用

页码：7363 / 7366

页数：4

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