Simplified ultrasensitive prion detection by recombinant PrP conversion with shaking

被引:228
作者
Atarashi, Ryuichiro [1 ,2 ]
Wilham, Jason M. [1 ]
Christensen, Leah [1 ]
Hughson, Andrew G. [1 ]
Moore, Roger A. [1 ]
Johnson, Lisa M. [1 ]
Onwubiko, Henry A. [1 ]
Priola, Suzette A. [1 ]
Caughey, Byron [1 ]
机构
[1] NIAID, Rocky Mt Labs, NIH, Persistent Viral Dis Lab, Hamilton, MT 59840 USA
[2] Nagasaki Univ, Grad Sch Biomed Sci, Dept Mol Microbiol & Immunol, Nagasaki 8528523, Japan
关键词
D O I
10.1038/nmeth0308-211
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:211 / 212
页数:2
相关论文
共 6 条
[1]   Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein [J].
Atarashi, Ryuichiro ;
Moore, Roger A. ;
Sim, Valerie L. ;
Hughson, Andrew G. ;
Dorward, David W. ;
Onwubiko, Henry A. ;
Priola, Suzette A. ;
Caughey, Byron .
NATURE METHODS, 2007, 4 (08) :645-650
[2]   In vitro generation of infectious scrapie prions [J].
Castilla, J ;
Saá, P ;
Hetz, C ;
Soto, C .
CELL, 2005, 121 (02) :195-206
[3]   Formation of native prions from minimal components in vitro [J].
Deleault, Nathan R. ;
Harris, Brent T. ;
Rees, Judy R. ;
Supattapone, Surachai .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (23) :9741-9746
[4]   CELL-FREE FORMATION OF PROTEASE-RESISTANT PRION PROTEIN [J].
KOCISKO, DA ;
COME, JH ;
PRIOLA, SA ;
CHESEBRO, B ;
RAYMOND, GJ ;
LANSBURY, PT ;
CAUGHEY, B .
NATURE, 1994, 370 (6489) :471-474
[5]   Ultra-efficient replication of infectious prions by automated protein misfolding cyclic amplification [J].
Saa, Paula ;
Castilla, Joaquin ;
Soto, Claudio .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (46) :35245-35252
[6]   Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding [J].
Saborio, GP ;
Permanne, B ;
Soto, C .
NATURE, 2001, 411 (6839) :810-813