Multiple CD4+ T Cell Subsets Produce Immunomodulatory IL-10 During Respiratory Syncytial Virus Infection

被引:88
作者
Weiss, Kayla A. [2 ]
Christiaansen, Allison F. [1 ]
Fulton, Ross B. [1 ]
Meyerholz, David K. [3 ]
Varga, Steven M. [1 ,2 ,3 ]
机构
[1] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA
[2] Univ Iowa, Interdisciplinary Grad Program Immunol, Iowa City, IA 52242 USA
[3] Univ Iowa, Dept Pathol, Iowa City, IA 52242 USA
基金
美国国家卫生研究院;
关键词
RECOMBINANT HUMAN INTERLEUKIN-10; CYTOKINE PRODUCTION; LUNG INFLAMMATION; US CHILDREN; IFN-GAMMA; MICE; EXPRESSION; IMMUNITY; INNATE; IMMUNOPATHOLOGY;
D O I
10.4049/jimmunol.1100764
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The host immune response is believed to contribute to the severity of pulmonary disease induced by acute respiratory syncytial virus (RSV) infection. Because RSV-induced pulmonary disease is associated with immunopathology, we evaluated the role of IL-10 in modulating the RSV-specific immune response. We found that IL-10 protein levels in the lung were increased following acute RSV infection, with maximum production corresponding to the peak of the virus-specific T cell response. The majority of IL-10-producing cells in the lung during acute RSV infection were CD4(+) T cells. The IL-10-producing CD4(+) T cells included Foxp3(+) regulatory T cells, Foxp3(-) CD4(+) T cells that coproduce IFN-gamma, and Foxp3(-) CD4(+) T cells that do not coproduce IFN-gamma. RSV infection of IL-10-deficient mice resulted in more severe disease, as measured by increased weight loss and airway resistance, as compared with control mice. We also observed an increase in the magnitude of the RSV-induced CD8(+) and CD4(+) T cell response that correlated with increased disease severity in the absence of IL-10 or following IL-10R blockade. Interestingly, IL-10R blockade during acute RSV infection altered CD4(+) T cell subset distribution, resulting in a significant increase in IL-17A-producing CD4(+) T cells and a concomitant decrease in Foxp3(+) regulatory T cells. These results demonstrate that IL-10 plays a critical role in modulating the adaptive immune response to RSV by limiting T-cell-mediated pulmonary inflammation and injury. The Journal of Immunology, 2011, 187: 3145-3154.
引用
收藏
页码:3145 / 3154
页数:10
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