Identification of new candidate vaccine antigens made by Streptococcus pyogenes:: Purification and characterization of 16 putative extracellular lipoproteins

被引：65

作者：

Lei, BF ^{[1
]}

Liu, MY ^{[1
]}

Chesney, GL ^{[1
]}

Musser, JM ^{[1
]}

机构：

[1] NIAID, Lab Human Bacterial Pathogenesis, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2004年 / 189卷 / 01期

关键词：

D O I：

10.1086/380491

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Putative extracellular lipoproteins made by group A Streptococcus ( GAS) are the focus of this study, which was designed to identify new candidate vaccine antigens. Bioinformatic analysis of a serotype M1 GAS strain identified 30 open-reading frames encoding putative lipoproteins. The genes encoding the mature form of 29 of these proteins were cloned, and 16 recombinant proteins were overexpressed in Escherichia coli and purified to apparent homogeneity. The genes encoding these 16 proteins were highly conserved in GAS strains for which genome sequence data are available (serotypes M1, M3, M5, M12, M18, and M28). Mice inoculated subcutaneously with GAS and humans with GAS pharyngitis and invasive infections seroconverted to most of the 16 recombinant proteins, which indicates that these lipoproteins were produced during infection. The blood of mice actively immunized with 5 of the 16 recombinant proteins had significantly (P<.05) increased growth-inhibitory activity, compared with the blood of unimmunized mice, which identified these proteins as potential new vaccine candidates.

引用

页码：79 / 89

页数：11

共 62 条

[1] Protection against group A streptococcus by immunization with J8-diphtheria toxoid: Contribution of J8-and diphtheria toxoid-specific antibodies to protection [J].

Batzloff, MR ;

Hayman, WA ;

Davies, MR ;

Zeng, M ;

Pruksakorn, S ;

Brandt, ER ;

Good, MF .

JOURNAL OF INFECTIOUS DISEASES, 2003, 187 (10) :1598-1608

[2] TYPE-SPECIFIC PROTECTIVE IMMUNITY EVOKED BY SYNTHETIC PEPTIDE OF STREPTOCOCCUS-PYOGENES M-PROTEIN [J].

BEACHEY, EH ;

SEYER, JM ;

DALE, JB ;

SIMPSON, WA ;

KANG, AH .

NATURE, 1981, 292 (5822) :457-459

[3] Genome sequence of a serotype M3 strain of group A Streptococcus:: Phage-encoded toxins, the high-virulence phenotype, and clone emergence [J].

Beres, SB ;

Sylva, GL ;

Barbian, KD ;

Lei, BF ;

Hoff, JS ;

Mammarella, ND ;

Liu, MY ;

Smoot, JC ;

Porcella, SF ;

Parkins, LD ;

Campbell, DS ;

Smith, TM ;

McCormick, JK ;

Leung, DYM ;

Schlievert, PM ;

Musser, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (15) :10078-10083

[4]

BESSEN D, 1990, J IMMUNOL, V145, P1251

[5] New multi-determinant strategy for a group A streptococcal vaccine designed for the Australian Aboriginal population [J].

Brandt, ER ;

Sriprakash, KS ;

Hobb, RI ;

Hayman, WA ;

Zeng, WG ;

Batzloff, MR ;

Jackson, DC ;

Good, MF .

NATURE MEDICINE, 2000, 6 (04) :455-459

[6] Immunization with components of two iron uptake ABC transporters protects mice against systemic Streptococcus pneumoniae infection [J].

Brown, JS ;

Ogunniyi, AD ;

Woodrow, MC ;

Holden, DW ;

Paton, JC .

INFECTION AND IMMUNITY, 2001, 69 (11) :6702-6706

[7] EXPRESSION VECTORS FOR AFFINITY PURIFICATION AND RADIOLABELING OF PROTEINS USING ESCHERICHIA-COLI AS HOST [J].

CHEN, BPC ;

HAI, TW .

GENE, 1994, 139 (01) :73-75

[8] Pathogenesis of group A streptococcal infections [J].

Cunningham, MW .

CLINICAL MICROBIOLOGY REVIEWS, 2000, 13 (03) :470-+

[9]

CUNNINGHAM MW, 1989, J IMMUNOL, V143, P2677

[10] Multivalent group A streptococcal vaccine designed to optimize the immunogenicity of six tandem M protein fragments [J].

Dale, JB .

VACCINE, 1999, 17 (02) :193-200

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