Molecular mechanisms mediating methylation-dependent silencing of ribosomal gene transcription

被引：195

作者：

Santoro, R ^{[1
]}

Grummt, I ^{[1
]}

机构：

[1] German Canc Res Ctr, Div Mol Biol & Cell 2, D-69120 Heidelberg, Germany

来源：

MOLECULAR CELL | 2001年 / 8卷 / 03期

关键词：

D O I：

10.1016/S1097-2765(01)00317-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epigenetic control mechanisms silence about half of ribosomal RNA genes (rDNA) in metabolically active cells. In the mouse, 40% of rDNA repeats are methylated and can be activated by 5-azacytidine treatment. In exploring the effect of methylation on rDNA transcription, we found that methylation of a single CpG dinucleotide within the upstream control element of the rDNA promoter (at -133) abrogates rDNA transcription both in transfection experiments and in in vitro assays using chromatin templates. Chromatin immunoprecipitation assays demonstrate that methylation of the cytosine at -133 inhibits binding of the transcription factor UBF to nucleosomal rDNA, thereby preventing initiation complex formation. Thus, methylation may be a mechanism to inactivate rDNA genes and propagate transcriptional silencing through cell division.

引用

页码：719 / 725

页数：7

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