Programmed adsorption and release of proteins in a microfluidic device

被引：481

作者：

Huber, DL ^{[1
]}

Manginell, RP ^{[1
]}

Samara, MA ^{[1
]}

Kim, BI ^{[1
]}

Bunker, BC ^{[1
]}

机构：

[1] Sandia Natl Labs, Albuquerque, NM 87185 USA

来源：

SCIENCE | 2003年 / 301卷 / 5631期

关键词：

D O I：

10.1126/science.1080759

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip.

引用

页码：352 / 354

页数：3

共 22 条

[1]

ACTIVE CONTROL OF WETTING USING APPLIED ELECTRICAL POTENTIALS AND SELF-ASSEMBLED MONOLAYERS [J].