Formation of electrostatic interactions on the protein-folding pathway

We describe a novel method of obtaining information about the structures of transient conformations on the folding pathway from their ionization equilibria: the H+-titration behavior of a protein residue is determined in detail by its environment. We follow the consolidation of electrostatic interactions in the folding process by comparing the acid-titration behavior of four conformations on the folding pathway of barnase: the denatured state (D); the folding intermediate (I); the major transition state (double dagger); and the native state (N) in the scheme D reversible arrow I reversible arrow double dagger reversible arrow N. The results show that strong electrostatic interactions are present in the major transition state: some of its carboxylate groups display the highly anomalous pK(A) values of <2 that are found in N. However, the network of ionic surface interactions is not formed in double dagger, and the overall protection of titrating residues is weakened. The results ale consistent with the transition state being an expanded form of the native state, with a weakened but poorly hydrated core and a loosened periphery. The surface residues in such an expanded conformation are, on average, farther apart than are those in the center of the molecule. The results concerning the folding intermediate are less clear cut. We show that the interpretation of kinetic data relating to folding intermediates depends critically on assumptions about their equilibrium with other denatured states. We have, however, characterized the pH and ionic strength dependence of an apparent stability of I, using the deviation from two-state folding behavior, which can be used to investigate electrostatic properties of folding intermediates from a variety of mechanisms. In general, the data imply that I is somewhat similar to double dagger. Apparently odd titration properties of I are investigated further in the accompanying paper [Oliveberg, M., & Fersht, A. (1996) Biochemistry 35, 2738-2749]. The approach in this study may be of particular use in testing theoretical results since the relationship between H+-titration properties and protein structure can be treated by classical electrostatics.