Antioxidant activity of propolis of various geographic origins

被引:657
作者
Kumazawa, S
Hamasaka, T
Nakayama, T
机构
[1] Univ Shizuoka, Lab Funct Food Sci, Shizuoka 4228526, Japan
[2] Univ Shizuoka, COE Program 21 Century, Sch Food & Nutr Sci, Shizuoka 4228526, Japan
关键词
antioxidant activity; propolis; free radical scavenging activity; LC/MS; PDA;
D O I
10.1016/S0308-8146(03)00216-4
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Propolis is a resinous substance collected by honeybees from various plant sources. The antioxidant activities of propolis of various geographic origins, i.e., Argentina, Australia, Brazil, Bulgaria, Chile, China (Hebei, Hubei, and Zhejiang), Hungary, New Zealand, South Africa, Thailand, Ukraine, Uruguay, United States, and Uzbekistan were compared. Ethanol extracts of propolis (EEP) were prepared and evaluated for antioxidant activities of EEP samples by the beta-carotene bleaching and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay systems. Major constituents were identified in EEP by HPLC analysis with photo-diode array (PDA) and mass spectrometric (MS) detection, and quantitatively analyzed. EEP from Argentina, Australia, China, Hungary and New Zealand had relatively strong antioxidant activities, and were also correlated with the total polyphenol and flavonoid contents. Propolis with strong antioxidant activity contained antioxidative compounds such as kaempferol and phenethyl caffeate. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:329 / 339
页数:11
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