Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp

被引：89

作者：

Rhee, Hyun-Woo ^{[1
]}

Lee, Chang-Ro ^{[2
]}

Cho, Seung-Hyon ^{[2
]}

Song, Mi-Ryung ^{[3
]}

Cashel, Michael ^{[4
]}

Choy, Hyon E. ^{[3
]}

Seok, Young-Jae ^{[2
]}

Hong, Jong-In ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Nat Resources, Dept Chem, Seoul 151747, South Korea

[2] Seoul Natl Univ, Dept Biol Sci, Seoul 151742, South Korea

[3] Chonnam Natl Univ, Sch Med, Dept Microbiol, Kwangju 501746, South Korea

[4] Natl Inst Hlth, NICHD, Mol Genet Lab, Bethesda, MD 20892 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 03期

关键词：

D O I：

10.1021/ja0759139

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes.

引用

页码：784 / +

页数：3

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