Alternating-laser excitation of single molecules

被引：251

作者：

Kapanidis, AN

Laurence, TA

Lee, NK

Margeat, E

Kong, XX

Weiss, S

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Physiol, Los Angeles, CA 90095 USA

[3] Lawrence Livermore Natl Lab, Phy Biosci Inst, Livermore, CA 94551 USA

来源：

ACCOUNTS OF CHEMICAL RESEARCH | 2005年 / 38卷 / 07期

关键词：

D O I：

10.1021/ar0401348

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single-molecule fluorescence spectroscopy addresses biological mechanisms and enables ultrasensitive diagnostics. We describe a new family of single-molecule fluorescence methods that uses alternating-laser excitation (ALEX) of diffusing or immobilized biomolecules to study their structure, interactions, and dynamics. This is accomplished using ratios that report on the distance between and the smichiometry of fluorophores attached to the molecules of interest. The principle of alternation is compatible with several time scales, allowing monitoring of fast dynamics or simultaneous monitoring of a large number of individual molecules.

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收藏

页码：523 / 533

页数：11

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