A fungal-responsive MAPK cascade regulates phytoalexin biosynthesis in Arabidopsis

被引:288
作者
Ren, Dongtao [1 ,2 ]
Liu, Yidong [1 ]
Yang, Kwang-Yeol [1 ]
Han, Ling [1 ]
Mao, Guohong [1 ]
Glazebrook, Jane [3 ,4 ]
Zhang, Shuqun [1 ]
机构
[1] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA
[2] China Agr Univ, State Key Lab Plant Physiol & Biochem, Beijing 100094, Peoples R China
[3] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA
[4] Univ Minnesota, Ctr Microbial & Plant Genom, St Paul, MN 55108 USA
关键词
defense signaling; fungal resistance; camalexin biosynthesis; Botrytis cinerea;
D O I
10.1073/pnas.0711301105
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Plant recognition of pathogens leads to rapid activation of MPK3 and MPK6, two Arabidopsis mitogen-activated protein kinases (MAPI(s), and their orthologs in other species. Here, we report that synthesis of camalexin, the major phytoalexin in Arabidopsis, is regulated by the MPK3/MPK6 cascade. Activation of MPK3/MPK6 by expression of active upstream MAPK kinase (MAPKK) or MAPKK kinase (MAPKKK) was sufficient to induce camalexin synthesis in the absence of pathogen attack. Induction of camalexin by Botrytis cinerea was preceded by MPK3/MPK6 activation, and compromised in mpk3 and mpk6 mutants. Genetic analysis placed the MPK3/ MPK6 cascade upstream of PHYTOALEXIN DEFICIENT 2 (PAD2) and PAD3, but independent or downstream of PAD1 and PAD4. Camalexin induction after MPK3/MPK6 activation was preceded by rapid and coordinated up-regulation of multiple genes encoding enzymes in the tryptophan (Trp) biosynthetic pathway, in the conversion of Trp to indole-3-acetaldoxime (IAOx, a branch point between primary and secondary metabolism), and in the camalexin biosynthetic pathway downstream of IAOx. These results indicate that the MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection.
引用
收藏
页码:5638 / 5643
页数:6
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