Baker's yeast mediated biotransformation of geraniol into citronellol using a continuous-closed-gas-loop bioreactor (CCGLB) system

被引：12

作者：

Arifin, Aimi Aishah ^{[1
]}

Don, Mashitah Mat ^{[1
]}

Uzir, Mohamad Hekarl ^{[1
]}

机构：

[1] Univ Sains Malaysia, Sch Chem Engn, Nibong Tebal 14300, Penang, Malaysia

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2011年 / 56卷 / 03期

关键词：

Yeast; Biotransformations; In-situ product removal; Continuous-closed-gas-loop bioreactor (CCGLB); Production kinetics; Heterogeneous biocatalysis; SACCHAROMYCES-CEREVISIAE; RHODOCOCCUS-ERYTHROPOLIS; ASYMMETRIC BIOREDUCTION; MEMBRANE BIOREACTOR; REDUCTION; LIQUID; CELLS; FERMENTATION;

D O I：

10.1016/j.bej.2011.07.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The kinetics of the biotransformation of geraniol into citronellol by the resting cells of Saccharomyces cerevisiae (baker's yeast type-II) was investigated in a continuous-closed-gas-loop bioreactor (CCGLB). Geraniol, which has high vapour pressure, high volatility and low solubility in aqueous medium, is highly suitable to be used in this system. Various operating parameters which affect the reduction reaction were investigated. The optimal conditions were; pH 7, agitation rate of 350 rpm, substrate flow rate of 8 L/min and glucose concentration of 50 g/L The gas loop led to a maximum citronellol concentration of 2.38 g/L with the specific activity of 7.90 U/g(cell), which were apparently higher than that of the values obtained using conventional systems. The CCGLB system also has the advantage of in situ product removal and can be readily utilized in large-scale production. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：219 / 224

页数：6

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