Haploidy breeding and mutagenesis for drought tolerance in wheat

被引:23
作者
Khan, AJ [1 ]
Hassan, S [1 ]
Tariq, M [1 ]
Khan, T [1 ]
机构
[1] NIFA, Tarnab Peshawar, Pakistan
关键词
anther culture; DH-mutants; doubled haploids; gamma rays; mutagenesis; mutants; Triticum aestivum;
D O I
10.1023/A:1017598202368
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Several intraspecific crosses between known drought tolerant wheat varieties and stable high yielding recombinants were made with the objective to develop improved cultivars for the moisture stressed rainfed areas of Pakistan. Five of these crosses were selected for further creation of useful mutations through the application of low doses of gamma rays and development of doubled haploids through anther culture. Anther culture response of the selected irradiated F-1 generations was studied on liquid and solid induction media. The highest number of calli among almost all crosses was produced on Potato-2 induction medium. All the crosses varied greatly in response to callus induction and maximum calli (75%) were obtained from Lyl-73/vee's' cross. Similarly, genotypic differences were found for green vs. albino regenerants. The highest number of green plantlets (12.1%) was recorded for Lu-26/3062. From the developed doubled haploid population 25 DH-mutants were initially selected and nine lines were finally included in multi-locational field tests. Two DH-mutants (i.e. DHML-50 and DHML-9) have potential for better grain yield, earliness, disease resistance and moisture stress tolerance.
引用
收藏
页码:409 / 414
页数:6
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