Acyl homoserine-lactone quorum-sensing signal generation

被引:390
作者
Parsek, MR
Val, DL
Hanzelka, BL
Cronan, JE
Greenberg, EP [3 ]
机构
[1] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA
[3] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA
关键词
D O I
10.1073/pnas.96.8.4360
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Acyl homoserine lactones (acyl-HSLs) are important intercellular signaling molecules used by many bacteria to monitor their population density in quorum-sensing control of gene expression. These signals are synthesized by members of the LuxI family of proteins, To understand the mechanism of acyl-HSL synthesis we have purified the Pseudomonas aeruginosa RhlI protein and analyzed the kinetics of acyl-HSL synthesis by this enzyme, Purified RhlI catalyzes the synthesis of acyl-HSLs from acyl-acyl carrier proteins and S-adenosylmethionine. An analysis of the patterns of product inhibition indicated that RhlI catalyzes signal synthesis by a sequential, ordered reaction mechanism in which S-adenosylmethionine binds to RhlI as the initial step in the enzymatic mechanism. Because pathogenic bacteria such as P. aeruginosa use acyl-HSL signals to regulate virulence genes, an understanding of the mechanism of signal synthesis and identification of inhibitors of signal synthesis has implications for development of quorum sensing-targeted antivirulence molecules.
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页码:4360 / 4365
页数:6
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