Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

被引:165
作者
Duchi, Serena [1 ,2 ]
Onofrillo, Carmine [2 ]
O'Connell, Cathal D. [2 ]
Blanchard, Romane [1 ]
Augustine, Cheryl [1 ]
Quigley, Anita F. [2 ,3 ,4 ]
Kapsa, Robert M. I. [2 ,3 ,4 ]
Pivonka, Peter [1 ]
Wallace, Gordon [2 ]
Di Bella, Claudia [1 ,2 ,5 ]
Choong, Peter F. M. [1 ,2 ,5 ]
机构
[1] Univ Melbourne, Dept Surg, St Vincents Hosp Melbourne, 29 Regent St Clin Sci Bldg, Fitzroy, Vic 3065, Australia
[2] Univ Wollongong, Intelligent Polymer Res Inst, ARC Ctr Excellence Electromat Sci, Innovat Campus, Wollongong, NSW 2522, Australia
[3] St Vincents Hosp, Dept Clin Neurosci, 5th Floor Daly Wing, Fitzroy, Vic 3065, Australia
[4] St Vincents Hosp Melbourne, Dept Med, Fitzroy, Vic 3065, Australia
[5] St Vincents Hosp Melbourne, Dept Orthopaed, Fitzroy, Vic 3065, Australia
基金
澳大利亚研究理事会;
关键词
HYDROGELS; BIOFABRICATION; FABRICATION; CONSTRUCTS;
D O I
10.1038/s41598-017-05699-x
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue engineering. Extrusion-based 3D bioprinting necessitates a phase change from a liquid bioink to a semi-solid crosslinked network achieved by a photo-initiated free radical polymerization reaction that is known to be cytotoxic. Therefore, the choice of the photocuring conditions has to be carefully addressed to generate a structure stiff enough to withstand the forces phisiologically applied on articular cartilage, while ensuring adequate cell survival for functional chondral repair. We recently developed a handheld 3D printer called " Biopen".To progress towards translating this freeform biofabrication tool into clinical practice, we aimed to define the ideal bioprinting conditions that would deliver a scaffold with high cell viability and structural stiffness relevant for chondral repair. To fulfill those criteria, free radical cytotoxicity was confined by a co-axial Core/Shell separation. This system allowed the generation of Core/Shell GelMa/HAMa bioscaffolds with stiffness of 200KPa, achieved after only 10 seconds of exposure to 700 mW/cm(2) of 365 nm UV-A, containing > 90% viable stem cells that retained proliferative capacity. Overall, the Core/Shell handheld 3D bioprinting strategy enabled rapid generation of high modulus bioscaffolds with high cell viability, with potential for in situ surgical cartilage engineering.
引用
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页数:12
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