Neuroprotective effect of recombinant adeno-associated virus human thioredoxin-PR39 on acute cerebral infarction in rats

被引：9

作者：

Guo, Yu-Dong ^{[1
]}

Huang, Teng ^{[2
]}

Sheng, Wen-Hua ^{[3
]}

Guan, Yun-Fei ^{[4
]}

Du, Yi-Feng ^{[5
]}

Lin, You-Ting ^{[5
]}

Ruan, Xi-Yun ^{[5
]}

机构：

[1] Fifth Peoples Hosp Jinan, Dept Neurol, Jinan 250022, Shandong, Peoples R China

[2] Shandong Univ, Shandong Prov Hosp, Dept Neurol, Jinan 250022, Shandong, Peoples R China

[3] Shandong Univ, Dept Neurol, Jinan Cent Hosp, Jinan 250013, Shandong, Peoples R China

[4] Shandong Univ, Sch Med, Dept Neurol, Jinan 250012, Shandong, Peoples R China

[5] Shandong Univ, Dept Neurol, Shandong Prov Hosp, 324 Jingwuweiqi Rd, Jinan 250021, Shandong, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2018年 / 16卷 / 03期

基金：

中国国家自然科学基金;

关键词：

adeno-associated virus; thioredoxin; PR39; gene fusion; gene therapy; acute cerebral infarction; ISCHEMIA-REPERFUSION INJURY; MEDIATED GENE-THERAPY; ANTIMICROBIAL PEPTIDE; ARTERY OCCLUSION; PR-39; STROKE; APOPTOSIS; PROLIFERATION; REGULATOR; BINDING;

D O I：

10.3892/etm.2018.6456

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

The recombinant adeno-associated virus human thioredoxin-PR39 (rAAV/hTRX-PR39) has been demonstrated to have a protective effect on hypoxic cells. The present study aimed to explore the potential effect of rAAV/hTRX-PR39 on acute cerebral infarction in rats. Middle cerebral artery occlusion (MCAO) model rats were produced and divided into three groups: Normal saline group, empty virus group (rAAV, without hTRX-PR39 cDNA) and rAAV/hTRX-PR39 group. Hematoxylin and eosin staining and electron microscopy observation were used to assess the morphological changes of ischemic brain tissue during different periods. Immunohistochemistry was employed to detect the expression of CD34 to reflect angiogenesis of ischemic brain tissue. Rats treated with rAAV/hTRX-PR39 showed an alleviated degree of ischemic brain edema relative to that in control groups, suggesting PR39 can ameliorate brain damage after cerebral ischemia. In the rAAV/hTRX-PR39 group, CD34-positive cells were significantly increased in ischemic brain tissues compared to control groups. Furthermore, CD34-positive cells were primarily observed around the perivascular in ischemic brain, indicating the angiogenesis role of PR39 in ischemic brain. The present findings suggest that PR39 could effectively ameliorate ischemic brain damage and promote angiogenesis, which may contribute to the treatment of acute cerebral infarction.

引用

页码：2633 / 2638

页数：6

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