An improved method to isolate primary human osteocytes from bone

被引：22

作者：

Bernhardt, Anne ^{[1
,2
]}

Wolf, Sophie ^{[1
,2
]}

Weiser, Emilia ^{[1
,2
]}

Vater, Corina ^{[1
,2
]}

Gelinsky, Michael ^{[1
,2
]}

机构：

[1] Tech Univ Dresden, Ctr Translat Bone Joint & Soft Tissue Res, Fetscherstr 74, D-01309 Dresden, Germany

[2] Univ Klinikum Dresden, Fetscherstr 74, D-01309 Dresden, Germany

来源：

BIOMEDICAL ENGINEERING-BIOMEDIZINISCHE TECHNIK | 2020年 / 65卷 / 01期

关键词：

collagenase digest; osteocalcin; osteocyte isolation; CELL-LINE; EXPRESSION;

D O I：

10.1515/bmt-2018-0185

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Osteocytes are of high importance in bone metabolism as they orchestrate bone remodeling, react to mechanosensory stimuli and have endocrine functions. In vitro investigations with osteocytes are therefore of high relevance for biomaterial and drug testing. The application of primary human cells instead of rodent osteocyte cell lines like MLOY4 and IDG SW3 is desirable but provides the challenge of isolating these cells, which are deeply embedded into the mineralized bone matrix. The present study describes an improved protocol for the isolation of human primary osteocytes. In contrast to an already established protocol, resting steps between the demineralization /digestion steps of the bone particles considerably improved the yield of osteocytes. Real-time polymerase chain reaction (PCR) analysis revealed the expression of typical osteocyte markers like osteocalcin, E11/podoplanin and dentin matrix protein 1 (DMP-1).

引用

页码：107 / 111

页数：5

共 13 条

[1]

Establishment and characterization of an osteocyte-like cell line, MLO-Y4 [J].