Modification of the double-disk test for detection of Enterobactetiaceae producing extended-spectrum and AmpC β-lactamases

被引：67

作者：

Pitout, JDD

Reisbig, MD

Venter, EC

Church, DL

Hanson, ND

机构：

[1] Univ Calgary, Div Microbiol, Calgary Lab Serv, Calgary, AB T3C 0J5, Canada

[2] Univ Calgary, Dept Pathol & Lab Med, Calgary, AB T3C 0J5, Canada

[3] Univ Orange Free State, Dept Med Microbiol, ZA-9300 Bloemfontein, South Africa

[4] Creighton Univ, Sch Med, Dept Med Microbiol & Immunol, Omaha, NE 68178 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 08期

关键词：

D O I：

10.1128/JCM.41.8.3933-3935.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Detection of extended-spectrum beta-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is pyoblematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized beta-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in Enterobacteriaceae.

引用

页码：3933 / 3935

页数：3

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