Structural insight into OprD substrate specificity

被引：83

作者：

Biswas, Shyamasri

Mohammad, M. Mohammad

Patel, Dimki R.

Movileanu, Liviu

van den Berg, Bert ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

[2] Syracuse Univ, Dept Phys, Syracuse, NY 13244 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2007年 / 14卷 / 11期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1038/nsmb1304

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

OprD proteins form a large family of substrate-specific outermembrane channels in Gram-negative bacteria. We report here the X-ray crystal structure of OprD from Pseudomonas aeruginosa, which reveals a monomeric 18-stranded beta-barrel characterized by a very narrow pore constriction, with a positively charged basic ladder on one side and an electronegative pocket on the other side. The location of highly conserved residues in OprD suggests that the structure represents the general architecture of OprD channels.

引用

收藏

页码：1108 / 1109

页数：2

相关论文

共 11 条

[1]

PRED-TMBB:: a web server for predicting the topology of β-barrel outer membrane proteins [J].

Bagos, PG ;

Liakopoulos, TD ;

Spyropoulos, IC ;

Hamodrakas, SJ .

NUCLEIC ACIDS RESEARCH, 2004, 32 :W400-W404

[2]

Function of Pseudomonas porins in uptake and efflux [J].

Hancock, REW ;

Brinkman, FSL .

ANNUAL REVIEW OF MICROBIOLOGY, 2002, 56 :17-38

[3]

The role of specific surface loop regions in determining the function of the imipenem-specific pore protein OprD of Pseudomonas aeruginosa [J].

Huang, HJ ;

Hancock, REW .

JOURNAL OF BACTERIOLOGY, 1996, 178 (11) :3085-3090

[4]

MEMBRANE TOPOLOGY AND SITE-SPECIFIC MUTAGENESIS OF PSEUDOMONAS-AERUGINOSA PORIN OPRD [J].

HUANG, HJ ;

JEANTEUR, D ;

PATTUS, F ;

HANCOCK, REW .

MOLECULAR MICROBIOLOGY, 1995, 16 (05) :931-941

[5]

An arginine ladder in OprP mediates phosphate-specific transfer across the outer membrane [J].

Moraes, Trevor F. ;

Bains, Manjeet ;

Hancock, Robert E. W. ;

Strynadka, Natalie C. J. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2007, 14 (01) :85-87

[6]

Role of the novel OprD family of porins in nutrient uptake in Pseudomonas aeruginosa [J].

Tamber, S ;

Ochs, MM ;

Hancock, REW .

JOURNAL OF BACTERIOLOGY, 2006, 188 (01) :45-54

[7]

TRIAS J, 1990, J BIOL CHEM, V265, P15680

[8]

Wolter DJ, 2004, FEMS MICROBIOL LETT, V236, P137, DOI [10.1016/j.femsle.2004.05.039, 10.1111/j.1574-6968.2004.tb09639.x]

[9]

Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa [J].

Yoshihara, E ;

Yoneyama, H ;

Ono, T ;

Nakae, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 247 (01) :142-145

[10]

Protein D2 porin of the Pseudomonas aeruginosa outer membrane bears the protease activity [J].

Yoshihara, E ;

Gotoh, N ;

Nishino, T ;

Nakae, T .

FEBS LETTERS, 1996, 394 (02) :179-182