Plant responses to ethylene gas are mediated by SCF EBF1/EBF2-dependent proteolysis of EIN3 transcription factor

被引:739
作者
Guo, HW [1 ]
Ecker, JR [1 ]
机构
[1] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA
关键词
D O I
10.1016/S0092-8674(03)00969-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plants use ethylene gas as a signal to regulate myriad developmental processes and stress responses. The Arabidopsis EIN3 protein is a key transcription factor mediating ethylene-regulated gene expression and morphological responses. Here, we report that EIN3 protein levels rapidly increase in response to ethylene and this response requires several ethylene-signaling pathway components including the ethylene receptors (ETR1 and EIN4), CTR1, EIN2, EIN5, and EIN6. In the absence of ethylene, EIN3 is quickly degraded through a ubiquitin/proteasome pathway mediated by two F box proteins, EBF1 and EBF2. Plants containing mutations in either gene show enhanced ethylene response by stabilizing EIN3, whereas efb1 efb2 double mutants show constitutive ethylene phenotypes. Plants over-expressing either F box gene display ethylene insensitivity and destabilization of EIN3 protein. These results reveal that a ubiquitin/proteasome pathway negatively regulates ethylene responses by targeting EIN3 for degradation, and pinpoint EIN3 regulation as the key step in the response to ethylene.
引用
收藏
页码:667 / 677
页数:11
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