Application of protein-fragment complementation assays in cell biology

被引：67

作者：

Remy, Ingrid ^{[1
]}

Michnick, Stephen W. ^{[1
]}

机构：

[1] Univ Montreal, Dept Biochim, Montreal, PQ H3C 3J7, Canada

来源：

BIOTECHNIQUES | 2007年 / 42卷 / 02期

关键词：

D O I：

10.2144/000112396

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology.

引用

页码：137 / +

页数：5

共 28 条

[1] A heterodimeric coiled-coil peptide pair selected in vivo from a designed library-versus-library ensemble [J].

Arndt, KM ;

Pelletier, JN ;

Müller, KM ;

Alber, T ;

Michnick, SW ;

Plückthun, A .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 295 (03) :627-639

[2]

CODY V, 1992, ANTI-CANCER DRUG DES, V7, P483

[3] A retrovirus-based protein complementation assay screen reveals functional AKT1-binding partners [J].

Ding, Zhiyong ;

Liang, Jiyong ;

Lu, Yiling ;

Yu, Qinghua ;

Zhou Songyang ;

Lin, Shiaw-Yih ;

Mills, Gordon B. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (41) :15014-15019

[4] β-Lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein-protein interactions [J].

Galarneau, A ;

Primeau, M ;

Trudeau, LE ;

Michnick, SW .

NATURE BIOTECHNOLOGY, 2002, 20 (06) :619-622

[5] Probing minimal independent folding units in dihydrofolate reductase by molecular dissection [J].

Gegg, CV ;

Bowers, KE ;

Matthews, CR .

PROTEIN SCIENCE, 1997, 6 (09) :1885-1892

[6] Antiparallel leucine zipper-directed protein reassembly: Application to the green fluorescent protein [J].

Ghosh, I ;

Hamilton, AD ;

Regan, L .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (23) :5658-5659

[7] Visualization of interactions among bZip and Rel family proteins in living cells using bimolecular fluorescence complementation [J].

Hu, CD ;

Chinenov, Y ;

Kerppola, TK .

MOLECULAR CELL, 2002, 9 (04) :789-798

[8] An improved mRFP1 adds red to bimolecular fluorescence complementation [J].

Jach, Guido ;

Pesch, Martina ;

Richter, Klaus ;

Frings, Sabine ;

Uhrig, Joachim F. .

NATURE METHODS, 2006, 3 (08) :597-600

[9] Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals [J].

Luker, KE ;

Smith, MCP ;

Luker, GD ;

Gammon, ST ;

Piwnica-Worms, H ;

Piwnica-Worms, DP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (33) :12288-12293

[10] Identifying off-target effects and hidden phenotypes of drugs in human cells [J].

MacDonald, Marnie L. ;

Lamerdin, Jane ;

Owens, Stephen ;

Keon, Brigitte H. ;

Bilter, Graham K. ;

Shang, Zhidi ;

Huang, Zhengping ;

Yu, Helen ;

Dias, Jennifer ;

Minami, Tomoe ;

Michnick, Stephen W. ;

Westwick, John K. .

NATURE CHEMICAL BIOLOGY, 2006, 2 (06) :329-337

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