Molded rigid polymer monoliths as separation media for capillary electrochromatography.: 2.: Effect of chromatographic conditions on the separation

被引：225

作者：

Peters, EC ^{[1
]}

Petro, M ^{[1
]}

Svec, F ^{[1
]}

Fréchet, JMJ ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 11期

关键词：

D O I：

10.1021/ac9713520

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The effect of chromatographic conditions on the performance of monolithic poly(butyl methacrylate-co-ethylene dimethacrylate-co-2-acrylamido-2-methyl-1-propanesulfonic acid) columns in capillary electrochromatography has been studied. The now velocity was found to be proportional to the strength of the electric field and both the pH and the composition of the mobile phase. A column efficiency of 120 000 plates/m at the optimum now velocity of 1.5 cm/min is achieved for all the monolithic capillary columns of identical composition and porosity, regardless of their length, which varied from 30 to 120 cm. The polymeric separation medium exhibits retention and selectivity properties similar to those of typical ODS packings for reversed-phase chromatography. In addition to the "classical" use of monolithic capillary columns for the electrochromatographic separation of small molecules in reversed-phase mode, larger styrene oligomers were also separated under isocratic elution conditions. In addition, the electroosmotically driven size exclusion chromatography of polystyrene standards with molecular weights up to 10(6) has been demonstrated for the first time.

引用

页码：2296 / 2302

页数：7

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