Improved isolation and crystallization of Photosystem I for structural analysis

被引：115

作者：

Fromme, P ^{[1
]}

Witt, HT ^{[1
]}

机构：

[1] Tech Univ Berlin, Max Volmer Inst Biophys Chem & Biochem, D-10623 Berlin, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1998年 / 1365卷 / 1-2期

关键词：

photosystem I; photosynthesis; crystallization; structure analysis; membrane protein;

D O I：

10.1016/S0005-2728(98)00059-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stable trimeric Photosystem I (PS I) was isolated from the cyanobacterium Synechococcus elongatus by use of stereochemically pure beta-dodecylmaltoside. Crystals of extremely pure PS I are grown by dialysis against low salt concentration. The improved PS I crystals were the basis for a PS I model derived from X-ray structure analysis at 4 Angstrom, resolution (Krauss et al., Nat. Struct. Biol. 3 (1996) 965-973; Schubert et al., J. Mel. Biol. 272 (1997) 741-769) and for detailed information on the electron transfer chain elaborated by techniques of magnetic resonance (H. Kass, Thesis, TU-Berlin, 1995; Kamlowski et al., Biochim. Biophys. Acta 1319 (1997) 188-198 and 199-213; Bittl et al., Biochemistry 36 (1997) 12001-12004). Crystallization procedures using micro- and macroseeding are described as basis for the forthcoming structure analysis. (C) 1998 Elsevier Science B.V.

引用

页码：175 / 184

页数：10

共 38 条

[1]

ADMAN ET, 1976, J BIOL CHEM, V251, P3801

[2] STRUCTURE OF THE REACTION CENTER FROM RHODOBACTER-SPHAEROIDES R-26 - THE COFACTORS .1. [J].

ALLEN, JP ;

FEHER, G ;

YEATES, TO ;

KOMIYA, H ;

REES, DC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) :5730-5734

[3] Pulsed EPR study of spin-coupled radical pairs in photosynthetic reaction centers: Measurement of the distance between P-700(center dot+) and A(1)(center dot-) in photosystem I and between P-865(center dot+) and Q(A)(center dot-) in bacterial reaction centers [J].

Bittl, R ;

Zech, SG .

JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (08) :1429-1436

[4] Pulsed EPR structure analysis of photosystem I single crystals: Localization of the phylloquinone acceptor [J].

Bittl, R ;

Zech, SG ;

Fromme, P ;

Witt, HT ;

Lubitz, W .

BIOCHEMISTRY, 1997, 36 (40) :12001-12004

[5] ORIGIN AND EARLY EVOLUTION OF PHOTOSYNTHESIS [J].

BLANKENSHIP, RE .

PHOTOSYNTHESIS RESEARCH, 1992, 33 (02) :91-111

[6] REFINED ANALYSIS OF THE TRIMERIC STRUCTURE OF THE ISOLATED PHOTOSYSTEM-I COMPLEX FROM THE THERMOPHILIC CYANOBACTERIUM SYNECHOCOCCUS SP [J].

BOEKEMA, EJ ;

DEKKER, JP ;

ROGNER, M ;

WITT, I ;

WITT, HT ;

VANHEEL, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 974 (01) :81-87

[7] EVIDENCE FOR A TRIMERIC ORGANIZATION OF THE PHOTOSYSTEM-I COMPLEX FROM THE THERMOPHILIC CYANOBACTERIUM SYNECHOCOCCUS SP [J].

BOEKEMA, EJ ;

DEKKER, JP ;

VANHEEL, MG ;

ROGNER, M ;

SAENGER, W ;

WITT, I ;

WITT, HT .

FEBS LETTERS, 1987, 217 (02) :283-286

[8]

CASTENHOLZ RW, 1988, METHOD ENZYMOL, V167, P68

[9] STRUCTURE OF RHODOPSEUDOMONAS-SPHAEROIDES R-26 REACTION CENTER [J].

CHANG, CH ;

TIEDE, D ;

TANG, J ;

SMITH, U ;

NORRIS, J ;

SCHIFFER, M .

FEBS LETTERS, 1986, 205 (01) :82-86

[10] FUNCTION AND ORGANIZATION OF PHOTOSYSTEM-I POLYPEPTIDES [J].

CHITNIS, PR ;

XU, Q ;

CHITNIS, VP ;

NECHUSHTAI, R .

PHOTOSYNTHESIS RESEARCH, 1995, 44 (1-2) :23-40

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