BLOS1, a putative BLOC-1 subunit, interacts with SNX1 and modulates root growth in Arabidopsis

被引：28

作者：

Cui, Yanyan ^{[2
,3
]}

Li, Xugang ^{[1
]}

Chen, Qingguo ^{[2
,3
]}

He, Xin ^{[3
]}

Yang, Qing ^{[2
,3
]}

Zhang, Aili ^{[2
,3
]}

Yu, Xin ^{[1
]}

Chen, Hao ^{[2
,3
]}

Liu, Naiyou ^{[3
]}

Xie, Qi ^{[3
]}

Yang, Weicai ^{[3
]}

Zuo, Jianru ^{[3
]}

Palme, Klaus ^{[1
]}

Li, Wei ^{[3
]}

机构：

[1] Univ Freiburg, Inst Biol 2, D-79104 Freiburg, Germany

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100101, Peoples R China

来源：

JOURNAL OF CELL SCIENCE | 2010年 / 123卷 / 21期

基金：

中国国家自然科学基金;

关键词：

Biogenesis of lysosome-related organelles complex 1 (BLOC-1); Sorting nexin 1 (SNX1); Auxin efflux transporter; Root growth; Endosomal transport; Vacuole; LYSOSOME-RELATED ORGANELLES; AUXIN EFFLUX CARRIER; ENDOCYTIC PATHWAY; EARLY ENDOSOMES; PROTEIN; TRANSPORT; DYSBINDIN; LOCALIZATION; TRAFFICKING; DEFINES;

D O I：

10.1242/jcs.069732

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Internalization and sorting of macromolecules are inherent properties of all eukaryotic cells that are achieved by vesicle trafficking. However, this process is relatively less understood in plants. An eight-subunit protein complex, BLOC-1, which is involved in endosomal transport from the endosomes to the lysosomes, has been identified in both human and mice. In this study, two homologous subunits of this complex, BLOS1 (or AtGCN5L1) and BLOS2, have been characterized in Arabidopsis. Both BLOS1 and BLOS2 interacted with SNX1 on the sorting endosomes. Inducible RNAi lines with reduced levels of BLOS1 had longer primary roots and more lateral roots. Consistently, PIN1 and PIN2 were increased in BLOS1 RNAi lines, implicating an impaired transport from the endosomes to the vacuoles. These results suggest that a putative BLOC-1 complex in Arabidopsis might mediate the vacuolar degradative transport through direct interaction with SNX1 to regulate the homeostasis of PIN1 and PIN2, which is important for plant growth and development.

引用

页码：3727 / 3733

页数：7

共 49 条

[1] Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism [J].