The active site structure of tetanus neurotoxin resolved by multiple scattering analysis in X-ray absorption spectroscopy

被引：20

作者：

Meneghini, C

Morante, S

机构：

[1] Univ Roma Tor Vergata, Dipartimento Fis, I-00133 Rome, Italy

[2] Ist Nazl Fis Nucl, Lab Nazl Frascati, I-00044 Frascati, Italy

[3] Ist Nazl Fis Mat, Sez B, Unita Roma Tor Vergata, Rome, Italy

[4] European Synchrotron Radiat Facil, Ist Nazl Fis Mat, F-38043 Grenoble, France

来源：

BIOPHYSICAL JOURNAL | 1998年 / 75卷 / 04期

关键词：

D O I：

10.1016/S0006-3495(98)77636-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A detailed study of the x-ray absorption spectrum of tetanus neurotoxin in the K-edge EXAFS region of the zinc absorber is presented that allows the complete identification of the amino acid residues coordinated to the zinc active site. A Very satisfactory interpretation of the experimental data can be given if multiple scattering contributions are included in the analysis. Comparing the absorption spectrum of tetanus neurotoxin to that of two other structurally similar zinc-endopeptidases, thermolysin and astacin, in which the zinc coordination mode is known from crystallographic data, we conclude that in tetanus neurotoxin, besides a water molecule, zinc is coordinated to two histidines and a tyrosine.

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页码：1953 / 1963

页数：11

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