Cloning, sequencing, and expression of an endoglucanase gene from the rumen anaerobic fungus Neocallimastix frontalis MCH3

被引：22

作者：

Fujino, Y

Ogata, K

Nagamine, T

Ushida, K ^{[1
]}

机构：

[1] Kyoto Prefectural Univ, Lab Anim Sci, Kyoto 6068522, Japan

[2] STAFF Inst, Tsukuba, Ibaraki 3050850, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 09期

关键词：

anaerobic fungi; Neocallimastix frontalis; endoglucanase; dockerin domain;

D O I：

10.1271/bbb.62.1795

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A cDNA clone encoding an endo-1,4-beta-glucanase from a rumen fungus, Neocallimastix frontalis MCH3, was isolated, The nucleotide sequence showed that the gene, celA, encoded a multidomain enzyme containing a family 5 catalytic domain and a reiterated sequence that is involved in the association of a multienzyme complex, the cellulosome. The enzyme expressed in Escherichia coli showed the highest activity against carboxymethylcellulose at 40 degrees C and pH 8.5.

引用

页码：1795 / 1798

页数：4

共 12 条

[1]

Akin DE, 1994, ANAEROBIC FUNGI BIOL, P169

[2]

PROTEOLYTIC ACTIVITY OF RUMEN FUNGI BELONGING TO THE GENERA NEOCALLIMASTIX AND PIROMYCES [J].