Metabolomics data reveal a crucial role of cytosolic glutamine synthetase 1;1 in coordinating metabolic balance in rice

被引:125
作者
Kusano, Miyako [2 ,3 ]
Tabuchi, Mayumi [1 ]
Fukushima, Atsushi [2 ]
Funayama, Kazuhiro [1 ]
Diaz, Celine [2 ]
Kobayashi, Makoto [2 ]
Hayashi, Naomi [2 ]
Tsuchiya, Yumiko N. [2 ,3 ]
Takahashi, Hideki [2 ,3 ]
Kamata, Atsuko [1 ]
Yamaya, Tomoyuki [1 ]
Saito, Kazuki [2 ,4 ]
机构
[1] Tohoku Univ, Grad Sch Agr Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan
[2] RIKEN Plant Sci Ctr, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
[3] Yokohama City Univ, Kihara Inst Biol Res, Totsuka Ku, Yokohama, Kanagawa 2440813, Japan
[4] Chiba Univ, Grad Sch Pharmaceut Sci, Inage Ku, Chiba 2638522, Japan
关键词
metabolite profiling; ammonium assimilation; cytosolic glutamine synthetase; rice; GC-TOF-MS; metabolic balance; AMMONIUM ASSIMILATION; SYNTHASE PROTEIN; NITROGEN; CARBON; RESPONSES; BIOSYNTHESIS; LOCALIZATION; POLYAMINES; SENESCENCE; INCREASE;
D O I
10.1111/j.1365-313X.2011.04506.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rice plants grown in paddy fields preferentially use ammonium as a source of inorganic nitrogen. Glutamine synthetase (GS) catalyses the conversion of ammonium to glutamine. Of the three genes encoding cytosolic GS in rice, OsGS1;1 is critical for normal growth and grain filling. However, the basis of its physiological function that may alter the rate of nitrogen assimilation and carbon metabolism within the context of metabolic networks remains unclear. To address this issue, we carried out quantitative comparative analyses between the metabolite profiles of a rice mutant lacking OsGS1;1 and its background wild type (WT). The mutant plants exhibited severe retardation of shoot growth in the presence of ammonium compared with the WT. Overaccumulation of free ammonium in the leaf sheath and roots of the mutant indicated the importance of OsGS1;1 for ammonium assimilation in both organs. The metabolite profiles of the mutant line revealed: (i) an imbalance in levels of sugars, amino acids and metabolites in the tricarboxylic acid cycle, and (ii) overaccumulation of secondary metabolites, particularly in the roots under a continuous supply of ammonium. Metabolite-to-metabolite correlation analysis revealed the presence of mutant-specific networks between tryptamine and other primary metabolites in the roots. These results demonstrated a crucial function of OsGS1;1 in coordinating the global metabolic network in rice plants grown using ammonium as the nitrogen source.
引用
收藏
页码:456 / 466
页数:11
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